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BEGIN:VEVENT
DTSTAMP:20260513T153412Z
UID:https://www.mpgc-mainz.de/events/34031/2368
DTSTART;VALUE=DATE:20230705
DTEND;VALUE=DATE:20230706
CLASS:PUBLIC
CREATED:20230323T144504Z
DESCRIPTION:Transcription involves many factors and chromatin elements that
  need to locally act in a coordinated fashion. However\, the underlying sp
 atiotemporal organization and regulation of these are incompletely underst
 ood. Recent work supports a model in which factors involved with transcrip
 tion can form phase-separated biomolecular condensates. Although these tra
 nscriptional condensates (TCs) are known to be large and dynamic multicomp
 onent clusters and thought to compartmentalize gene expression\, little is
  known about their properties and functions in <i>live</i> cells. Here\, w
 e set out to establish a platform for studying transcriptional condensates
  directly in live cells under near-physiological conditions. We use multig
 ene endogenous fluorescent tagging with high-throughput live-cell and supe
 r-resolution imaging techniques to determine TC properties. In combination
  with systematic perturbations\, we characterize TCs\, elucidate factor an
 d chromatin dependencies\, and investigate how TCs can react to their envi
 ronment. Our investigations show that in response to environmental changes
  as well as specific cellular perturbations the abundance\, size\, and dyn
 amics of TCs are altered. These experiments also unravel novel and unantic
 ipated dependencies\, which we now want to understand in more detail. In c
 onclusion\, our live cell and endogenous approach enables a thorough chara
 cterization of transcriptional condensates. The results obtained have prov
 ided insights into the dynamic properties of TCs and are thereby relevant 
 to the regulation of gene expression in space and time.\nSpeaker: Samuel S
 houp
LAST-MODIFIED:20230414T072109Z
LOCATION:
SUMMARY:Live-cell characterization of transcriptional condensates
URL;VALUE=URI:https://www.mpgc-mainz.de/events/34031/2368
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